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ebm-2 ec growth medium 00190860  (Lonza)


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    Structured Review

    Lonza ebm-2 ec growth medium 00190860
    Ebm 2 Ec Growth Medium 00190860, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ec+growth+medium+(ebm-2/ebm+2+medium/pmc11549335-527-7-12
    Average 90 stars, based on 1 article reviews
    ebm-2 ec growth medium 00190860 - by Bioz Stars, 2026-10
    90/100 stars

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    Cell Culture:

    Article Title: MARTX toxin of Vibrio vulnificus induces RBC phosphatidylserine exposure that can contribute to thrombosis
    Article Snippet: Human umbilical vein endothelial cells were originally purchased from Lonza (C2517A). The RBCs pre-infected with V. vulnificus for 30 min were moved to human umbilical vein endothelial cells (ECs, 2 × 10 4 cells) pre-stained with Calcein green (Invitrogen) and then incubated at 37 °C for 30 min. The RBCs pre-infected but not adhered to ECs were washed out using the EC growth medium (EBM-2 (Lonza)). The RBCs pre-infected and adhered to ECs were stained with the anti-glycophorin-A-PE (BD Bioscience, 555570, 1:100 dilution) and then analyzed by fluorescence microscopy (Axiovert 200 M, Zeiss). To observe self-aggregation, the RBCs were infected with V. vulnificus , stained with the anti-glycophorin-A-PE (BD Bioscience, 555570, 1:100 dilution), and then analyzed by

    Article Title: Krüppel-like factor 3 inhibition by mutated lncRNA Reg1cp results in human high bone mass syndrome
    Article Snippet: mononuclear cells were isolated from blood by density gradient centrifugation with Histopaque-1077 (10771; Sigma-Aldrich). .. Mononuclear cells were plated in 0.5 ml EC growth medium (EBM-2; Clonetics; Lonza) supplemented with EGM-2 Single Quots (CC-4176; Clonetics; Lonza) on fibronectin/gelatin–coated 24-well plates. .. After 7 d of culture, the attached endothelia cells were collected for RNA extraction.



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    KEY RESOURCES TABLE

    Journal: Neuron

    Article Title: Motor neurons use push-pull signals to direct vascular remodeling critical for their connectivity

    doi: 10.1016/j.neuron.2022.09.021

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Cell lines Human umbilical vascular ECs (HUVEC; Lonza Cat# C2519A) were cultured in EC Growth Basal Medium-2 (EBM-2; Lonza Cat# CC-3156) supplemented with EGM-2 Endothelial factors (Lonza Cat#CC-4176).

    Techniques: Recombinant, Protease Inhibitor, Transfection, Plasmid Preparation, Cloning, Mutagenesis, Reverse Transcription, Sample Prep, RNA Library Preparation, Knock-Out, Control, Quantitative RT-PCR, In Situ, Software, Expressing, CRISPR, RNAscope

    KEY RESOURCES TABLE

    Journal: Neuron

    Article Title: Motor neurons use push-pull signals to direct vascular remodeling critical for their connectivity

    doi: 10.1016/j.neuron.2022.09.021

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Cell lines Human umbilical vascular ECs (HUVEC; Lonza Cat# C2519A) were cultured in EC Growth Basal Medium-2 (EBM-2; Lonza Cat# CC-3156) supplemented with EGM-2 Endothelial factors (Lonza Cat#CC-4176).

    Techniques: Recombinant, Protease Inhibitor, Transfection, Plasmid Preparation, Cloning, Mutagenesis, Reverse Transcription, Sample Prep, RNA Library Preparation, Knock-Out, Control, Quantitative RT-PCR, In Situ, Software, Expressing, CRISPR, RNAscope